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KMID : 1134820140430030419
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Journal of the Korean Society of Food Science and Nutrition
2014 Volume.43 No. 3 p.419 ~ p.424
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Metabolomic Analysis of Ethyl Acetate and Methanol Extracts of Blueberry
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Jo Young-Hee
Kim Su-Gyeong
Kwon Da-Ae
Lee Hong-Jin
Choi Hyung-Kyoon
Auh Joong-Hyuck
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Abstract
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Metabolite profiling of blueberry (cultivar "Spartan") was performed by extraction using different solvents, methanol and ethyl acetate, through metabolomic analysis using LC-MS/MS. Unsupervised classification method (PCA) and supervised prediction model (OPLS-DA) provided good categorization of metabolites according to the extraction solvents. Metabolites of the anthocyanin family, including delphinidin hexoside, delphinidin, 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, and petunidin hexoside, were mainly detected in methanol fractions, whereas those of the flavonoid family, including chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosyl-luteolin, and luteolin were successfully prepared in the ethyl acetate fraction. Thus, metabolomic analysis of blueberry extracts allows for the simple profiling of whole and distinctive metabolites for future applications.
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KEYWORD
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metabolites profiling, blueberry, LC-MS/MS
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